Accession Number : AD0625726

Title :   MECHANISM OF UTILIZATION OF CARBON SOURCES BY FLAGELLATED PROTOZOA.

Descriptive Note : Final rept.,

Corporate Author : CALIFORNIA UNIV LOS ANGELES DEPT OF ZOOLOGY

Personal Author(s) : Levedahl,Blaine H.

Report Date : 1965

Pagination or Media Count : 2

Abstract : The transport of succinate into Euglena gracilis var bacillaris (streptomycin bleached) was investigated with the use of structural analogs and metabolic inhibitors. The study showed a transport system different than that used for acetate. The transport system is judged to be a stereo-specific site requiring two carboxyl groups in a cis position separated by no more than three carbon atoms and two intact hydrogen atoms in the carbon position. Studies were begun on identification of the first products formed after succinate uptake. Coupled with this work was the finding that Euglena fixes rather large amounts of C02 heterotrophically when succinate is used as a carbon source but not, apparently, when acetate is the sole carbon source. Results of experiments using labeled succinate and non-labeled C02 when compared with those using labeled C02 and non-labeled succinate, indicate that C02 may be intimately connected with succinate utilization by Euglena. With the finding of significant heterotrophic C02 fixation by Euglena (bleached), a study was undertaken to find an effective method for sub-cellular fractionation of Euglena to permit study of the enzyme systems involved in the fixation process. (Author)

Descriptors :   (*SUCCINATES, PROTOZOA), (*PROTOZOA, SUCCINATES), ANTIMETABOLITES, TRANSPORT PROPERTIES, CARBON, CARBON DIOXIDE, LABELED SUBSTANCES, ENZYMES, METABOLISM

Subject Categories : Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE