Accession Number : AD0627055

Title :   STUDIES ON THE MECHANISM OF BIOGENIC AMINE RELEASE.

Descriptive Note : Annual technical rept. no. 1, 1 Sep 64-30 Nov 65,

Corporate Author : SAO PAULO UNIV (BRAZIL) SCHOOL OF MEDICINE OF RIBEIRAO PRETO

Personal Author(s) : Rothschild,Adolfo Max

Report Date : DEC 1965

Pagination or Media Count : 20

Abstract : Effects of inhibitors of the Embden-Meyerhof pathway of glycolysis as well as effects of inhibitors of glucose transport across cell membranes on the release of histamine from rat tissues exposed to a 'chemical' releaser (Compound 48-80) are studied. Potentiating effects of 2-4-dinitrophenol and of epinephrine on the spontaneous granule and histamine discharge occurring in rat tissue 'in-vitro' are also examined. It is concluded that in order to release histamine, mast cells must be able to draw upon metabolic stores which can be degraded by an anaerobic pathway, most probably glycolysis. Investigations about the role of phospholipase A on the histamine releasing response of rat mast cells revealed that this enzyme requires the presence of extracellular phospholipid substrates and is probably not involved in mast cell response to compound 48/80. An examination of the relative importance of histamine and bradykinin on the production of the symptoms of passive cutaneous anaphylaxis in the rat, revealed that the latter is the mediator of this reaction. The experimental approach which yielded this result, involved the use of sulfated polysaccharides as activators of the bradykinin-releasing system of plasma. (Author)

Descriptors :   (*HISTAMINE, METABOLISM), (*GLYCOLYSIS, INHIBITION), GLUCOSE, TRANSPORT PROPERTIES, CELLS(BIOLOGY), MEMBRANES(BIOLOGY), TISSUES(BIOLOGY), EPINEPHRINE, SEROTONIN, VENOMS, IN VITRO ANALYSIS, PHOSPHOLIPIDS, ENZYMES, ANAPHYLAXIS, SKIN(ANATOMY), POLYSACCHARIDES, SULFATES, CHROMATOGRAPHIC ANALYSIS, BLOOD PLASMA, BRAZIL

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE