Accession Number : AD0627366

Title :   NON-ENZYMIC PRODUCTION OF SOLUBLE COLORED PRODUCTS FROM INSOLUBLE PROTEIN-DYE SUBSTRATES,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Bobbitt,Jesse L. ; Luster,Philip L.

Report Date : 30 SEP 1965

Pagination or Media Count : 22

Abstract : Insoluble protein-dye complexes, such as high powder dyed with Congo red (congocoll), are useful substrates for assaying proteinases. Hydrolysis of the protein releases soluble colored products which can be measured readily. Soluble colored products also can be produced by solutions containing proteins having a strong affinity for the non -covalently bound dye of the substrate. In such cases, false indications of proteolytic activity are obtained through the non-enzymic formation of soluble protein-dye complexes. The non-enzymic process can be detected by changes in the absorption spectrum and by differences in the kinetics of color formation in the presence of excess liquid phase. Enzymic degradation of the insoluble substrates is proportional to both the concentration of the enzyme and the interfacial area of the substrate. Non -enzymic color production is determined by the total amount of soluble protein and does not increase with time. Non-enzymic contributions can be minimized by use of sufficient substrate or with blanks containing suitably inactivated enzyme. (Author)

Descriptors :   (*PEPTIDE HYDROLASES, BIOASSAY), (*BIOASSAY, DYES), (*DYES, COMPLEX COMPOUNDS), (*BIOLOGICAL STAINS, PEPTIDE HYDROLASES), (*PROTEINS, BIOLOGICAL STAINS), SERUM ALBUMIN, COLORS, ABSORPTION, PH FACTOR, TRYPSIN

Subject Categories : Biochemistry
      Polymer Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE