Accession Number : AD0627367

Title :   INTERACTIONS BETWEEN CORTISOL OR CORTICOSTERONE AND FRACTIONS OF RAT THYMUS, BRAIN AND HEART CELL,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : DeVenuto,Frank ; Chader,Gerald

Report Date : 17 SEP 1965

Pagination or Media Count : 19

Abstract : Interactions between (4-14C) cortisol or (4-14C) corticosterone and fractions of thymus, brain and heart cells of normal rats have been studied by multiple equilibrium dialysis; animals of different age, ranging from weaned (20 days of age) to adult (45 days) to old rats (100 days and over) have been used. Binding between the steroid hormones and total homogenates of thymus, brain and heart of the rat has also been investigated. The combining affinity for the two steroids with cytoplasmic fractions is greater than with nuclear fractions, especially for thymus and heart cell. The binding of corticosterone with all the fractions and the total homogenates obtained from animals of different age is higher than that observed with cortisol. A characteristic pattern showing a low binding affinity for 45 days old rats is observed in the interaction between the steroid hormones and some fractions for animals of different age. No accumulation of radioactive material was found in any particular fraction of the tissues investigated. (Author)

Descriptors :   (*CORTICOSTEROID AGENTS, TISSUES(BIOLOGY)), (*CYTOCHEMISTRY, CORTICOSTEROID AGENTS), THYMUS, BRAIN, HEART, CELLS(BIOLOGY), STEROIDS, HORMONES, CYTOPLASM, PROTEINS, RATS

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE