Accession Number : AD0631145

Title :   THE DNA CONTENT OF MITOTIC NUCLEI OF TWO MOUSE TUMORS,

Corporate Author : NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s) : Alpen,E. L. ; Johnston,M. E.

Report Date : 03 MAR 1966

Pagination or Media Count : 18

Abstract : A new high-precision microspectrophotometric method was used to test the validity of the 'DNA constancy hypothesis' in two mammalian tumor lines. The two are the mouse ascitic tumor BP-8 and the in vitro mouse leukemic cell line, L5178Y. It was shown that the mitotic nuclei of these cells have a DNA content with a coefficient of variation of 5-6%. The instrumental and measurement errors are 4-5%; thus the variability of DNA content as measured by absorption of Feulgen stain is 1% or less. (Author)

Descriptors :   (*DEOXYRIBONUCLEIC ACIDS, *NEOPLASMS), RADIATION EFFECTS, CELLS(BIOLOGY), NUCLEI(BIOLOGY), LEUKEMIA, BIOCHEMISTRY, MICE, MICROPHOTOMETERS, SPECTROPHOTOMETERS, MITOSIS

Subject Categories : Biochemistry
      Medicine and Medical Research
      Radiobiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE