Accession Number : AD0633970

Title :   RED BLOOD CELL PRESERVATION.

Descriptive Note : Progress rept. for 1 Jul 65-30 Jun 66,

Corporate Author : VIRGINIA UNIV CHARLOTTESVILLE BIOCHEMICAL LAB

Personal Author(s) : Chanutin,Alfred

Report Date : 30 JUN 1966

Pagination or Media Count : 64

Abstract : A number of erythrocyte phosphate metabolites (adenosine tri- and diphosphates, guanosine triphosphate, 2,3-diphosphoglycerate) affected heme-heme interaction and oxygen affinity with hemoglobin which resulted in a shift of the oxyhemoglobin dissociation curve to the right. In addition, tripolyphosphate, and tetra- and hexametaphosphates, particularly the latter, affected the dissociation curve in the same manner. Adenosine monophosphate and ribose-5-phosphate had no effect. These results, which are preliminary, were obtained with hemolysates and purified hemoglobin. Bloods, collected in ACD (pH 5.0) and CPD (pH 5.75) with and without additives, were stored in the cold (4C) for six weeks. After 0,2,4 and 6 weeks storage, the erythrocytes were assayed for DPG, ATP, ADP, AMP, IMP, TPN and GTP by ion exchange chromatography. The ATP of red cells in stored unsupplemented blood was maintained at higher concentrations than those stored in CPD blood; DPG levels were greater in CPD blood. These differences were due to the pH of the blood. The remaining compounds were not appreciably changed during storage. In ACD blood supplemented with small amounts of adenine (0.05 to 0.10 mmole/100 ml), the ATP concentrations were higher than in those which contained greater amounts of adenine (0.5 mmole). The DPG values decreased rapidly in these bloods. (Author)

Descriptors :   (*ERYTHROCYTES, PRESERVATION), BLOOD, ADENOSINE PHOSPHATES, OXYGEN, HEMOGLOBIN, PHOSPHATES, ADDITIVES, STORAGE, TEMPERATURE, CHROMATOGRAPHIC ANALYSIS, PH FACTOR

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE