Accession Number : AD0634018

Title :   THE EFFECT OF DIPYRIDAMOLE ON THE ADENOSINE TRIPHOSPHATE LEVEL OF STORED HUMAN BLOOD.

Descriptive Note : Progress rept. 15 Feb-30 Sep 65,

Corporate Author : BOSTON UNIV MASS SCHOOL OF MEDICINE

Personal Author(s) : Gibson,John G. ; Lionetti,Fabian

Report Date : 30 SEP 1965

Pagination or Media Count : 29

Abstract : The addition of dipyridamole, a synthetic deaminase inhibitor at a concentration of .0001 M to human Heparin-ACD blood fortified with adenosine, resulted in an increase in ATP above control levels during storage at 4 C, rising to a maximum of 130% at five days and falling to 20% at 30 days. Aliquots of the same blood fortified with adenine, adenosine or both incubated at 37 C for 30 hours showed levels at or slightly above the upper limit of +2 SD of normal. The addition of dipyridamole at a concentration of .0001M to similar aliquots showed significant increases in ATP above normal, rising to a maximum of 300, 350 and 400% in the aliquots fortified with adenine, adenosine and both respectively. Whereas the presence of adenine, adenosine or both in Heparin-ACD blood did not significantly alter the rise in whole blood inorganic phosphate during incubation at 37 C for 30 hours, the addition of dipyridamole to similar aliquots depressed the level by from 20 to 30% of control values. Protection of the amino group of the adenine constituent of adenosine and AMP is a means to conserve erythrocyte ATP. (Author)

Descriptors :   (*BLOOD, STORAGE), (*ERYTHROCYTES, PRESERVATION), (*ENZYME INHIBITORS, ADENOSINE PHOSPHATES), (*ADENOSINE PHOSPHATES, BLOOD), ADENINE, AMINO ACIDS, PH FACTOR, TEMPERATURE

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE