Accession Number : AD0634405

Title :   EFFECTS OF RADIATION ON THE SUBMICROSCOPIC STRUCTURE OF THE SYMPATHETIC NEURON.

Descriptive Note : Final rept. 16 Mar 62-15 Jul 64,

Corporate Author : BATTELLE MEMORIAL INST COLUMBUS OHIO

Personal Author(s) : ROPPEL, Richard M. ; Melton,Carl W. ; Peters,Arthur C. ; Allton,Wayne H. ; Prince,John E.

Report Date : APR 1966

Pagination or Media Count : 17

Abstract : Hamsters were irradiated with supralethal, single doses and with sublethal, fractionated doses of Co60 gamma radiation. Autonomic ganglia were dissected from these animals at various times after irradiation and were studied by electron microscopy for evidence of morphologic change resulting from irradiation. When fixation was accomplished by direct immersion of tissues in permanganate solution, only minimal cellular changes that could be attributed to radiation were observed in sympathetic tissues following single massive doeses of 10,000 to 100,000 r. When perfusion fixation was attempted, however, extensive fixation artifact was observed in the tissues of irradiated animals but not in controls. Fractionated-dose irradiation to a total of 500 r administered as ten 50 r doses resulted in an increase in the number of densely staining, cytoplasmic inclusion bodies observed in sympathetic neurons. A perman-ganate-sodium plumbate staining procedure which results in an electron microscope image of high contrast is described. (Author)

Descriptors :   (*NERVE CELLS, RADIATION EFFECTS), (*AUTONOMIC NERVOUS SYSTEM, RADIATION EFFECTS), CELL STRUCTURE, GANGLIA, HAMSTERS, DOSAGE, ELECTRON MICROSCOPY, TISSUES(BIOLOGY), GAMMA RAYS, MORPHOLOGY(BIOLOGY)

Subject Categories : Radiobiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE