Accession Number : AD0634636

Title :   REGULATION OF METABOLISM, IN VIVO,

Corporate Author : EDGEWOOD ARSENAL MD

Personal Author(s) : Sacktor, Bertram ; wormser-Shavit,E.

Report Date : JUN 1966

Pagination or Media Count : 11

Abstract : The concentrations of 35 critical metabolites, including glycogen, trehalose, components of the glycolytic pathway and citric acid cycle, amino acids, phosphates, and adenine nucleotides in flight muscle of the flowfly, Phormia, were measured concurrently after periods of flight ranging from 5 sec. to 1 hr. Coincident changes in metabolites concomitant with the 100-fold increase in glycolytic flux attained on initiation of flight provided an unique system to determine the steps that control glycolysis in vivo, in the transition from a tissue at rest to one performing strenuous work. Three sites of regulation: the phosphorylation of fructose-6-phosphate; the cleavage of trehalose; and the phosphorolysis of glycogen were established in this system in vivo. Additional controls at the oxidations of alpha-glycerophosphate and of proline are suggested. (Author)

Descriptors :   (*METABOLISM, IN VIVO ANALYSIS), BIOCHEMISTRY, GLYCOGEN, GLYCOLYSIS, AMINO ACIDS, PHOSPHATES, NUCLEOTIDES, SACCHARIDE PHOSPHATES, PHOSPHORYLATION, OXIDATION, STRESS(PHYSIOLOGY), TISSUES(BIOLOGY), DIPTERA, FLIGHT, INSECTS

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE