Accession Number : AD0634639

Title :   RED CELL FRAGILITY AND BLOOD PRESERVATION - THE OSMOGRAM,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Shields ,Charles E. ; Camp,Frank R. , Jr

Report Date : JUN 1966

Pagination or Media Count : 15

Abstract : Testing of osmotic fragility in blood stored for various intervals demonstrated an increased tendency to hemolyze in hypotonic solutions. Though not evident immediately after severe experimental trauma, the results showed diminished ability to survive during storage. In the osmogram method employed in this study, certain values were found to be more sensitive indicators of osmotic fragility. In addition to those recipients receiving stored blood in autotransfusions, the post-transfusion survival percentage could be correlated with osmotic fragility tests. In this study, the slope value measurements approaching the value of 1.00 were found in those having the better post-transfusion percentages. Application of these findings may be useful in predicting the ability of various stored blood units to survive in the recipient. The method may also be applied in comparing preservation in units after use of various additives. Finally, the blood bank may screen for units still useful for transfusion prior to expiration leading to better conservation of the supply of stored blood.

Descriptors :   (*ERYTHROCYTES, PRESERVATION), HEMOLYSIS, OSMOSIS, HEMOGLOBIN, MEASUREMENT, SPECTROPHOTOMETERS, ADDITIVES, BLOOD TRANSFUSION, STORAGE, TEST METHODS

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE