Accession Number : AD0635262

Title :   FLUORESCENCE MICROSCOPY STUDIES OF CELLULAR CHANGES IN CARCINOGENESIS.

Descriptive Note : Master's thesis.

Corporate Author : INDIANA UNIV INDIANAPOLIS SCHOOL OF DENTISTRY

Personal Author(s) : Krolls, Sigurds O.

Report Date : 1966

Pagination or Media Count : 177

Abstract : The study was designed to evaluate the progressive cellular changes in induced carcinomas in the cheek pouches and the skin in hamsters, utilizing fluorescence and the light microscopy in the examination of tissue and cytologic specimens. Sixty male syrian golden hamsters approximately four weeks old were divided into two equal experimental and control groups. The experimental group was given topical application of 0.5% dimethylbenz anthracene in mineral oil every second day to both cheek pouches and the interscapular area. The control group received similar applications of the vehicle utilized in this study, Mineral oil, heavy grade, (USP). Results indicated that, in the cytologic smears, the fluorescence increases in brightness before any morphologic changes were observed in the Papanicolaou prepared smears and is presumed to be associated with the increase in the nucleic acid concentration before cell division. The tissue sections stained with the fluorochromes revealed the relative concentration of the nucleic acids. The concentration increased in the basal cell layer of the epithelium as the experiment progressed. The cells were brightly fluorescent in the dyskeratotic stage, but as a well differentiated, slow growing epidermoid carcinoma developed epidermoid carcinomas, it became apparent that the thickness of the epithelium and the presence of the adnexal structures influenced the length of the latent period. In this experiment the skin lesions and the lesions at the orifice of the pouch developed before true buccal pouch lesions were observed. (Author)

Descriptors :   (*CANCER, MOUTH), (*CYTOLOGY, CANCER), HISTOLOGY, BIOPSY, PATHOLOGY, MORPHOLOGY(BIOLOGY), CELLS(BIOLOGY), TISSUES(BIOLOGY), CELL DIVISION, NUCLEIC ACIDS, POLYCYCLIC COMPOUNDS, HYDROCARBONS, ENZYMES, HORMONES, VIRUSES, NUTRITION, GENETICS, FLUORESCENCE, MICROSCOPY, PHOTOMICROGRAPHY, SKIN(ANATOMY), HAMSTERS, DENTISTRY

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE