Accession Number : AD0635929

Title :   MECHANISM OF MICROBIOLOGICAL CONTAMINATION OF JET FUEL AND DEVELOPMENT OF TECHNIQUES FOR DETECTION OF MICROBIOLOGICAL CONTAMINATION.

Descriptive Note : Semiannual progress rept., 1 Jan-1 Jun 65.

Corporate Author : MELPAR INC FALLS CHURCH VA

Personal Author(s) : Blanchard, Gordon C. ; Goucher,Charles R.

Report Date : JUL 1966

Pagination or Media Count : 82

Abstract : Two methods for detecting microorganisms were examined in the light of the present understanding of the jet fuel microorganisms. One method was based on a color produced by the enzymes of the organism. This method was found suitable for the detection of organisms in the early part of their growth in fuel systems. However, the sensitivity of the method declined slightly with the age of the culture. Another detection method was devised, tested, and found to be highly sensitive and independent of the age of the culture. This detection method has two simple steps. The first step is the reaction of microbial cells with a specific metal ion; the second step is the reaction of bound metal ions with a colored reagent. The method promises to yield a simple visual readout system capable of field application. The production of compounds by microorganisms associated with aluminum corrosion was dependent on the presence of high concentrations of nitrate ion as well as on jet fuel components. A direct relationship was found between the onset time of respiratory decline and the appearance of corrosive pigments in microbial cultures oxidizing jet fuel. (Author)

Descriptors :   (*BIOLOGICAL CONTAMINATION, *JET ENGINE FUELS), (*FUEL CONTAMINATION, MICROORGANISMS), DETERMINATION, CULTURE MEDIA, FUNGI, CORROSION, OXIDATION, FUNGUS DETERIORATION, ALUMINUM ALLOYS

Subject Categories : Microbiology
      Fuels

Distribution Statement : APPROVED FOR PUBLIC RELEASE