Accession Number : AD0637797

Title :   TRACE PEROXY COMPONENTS AND IMPURITIES IN INTRAVENOUS FAT EMULSIONS.

Descriptive Note : Rept. for 1 Feb-1 Jul 66.

Corporate Author : APPLIED SCIENCE LABS INC STATE COLLEGE PA

Personal Author(s) : Pelick,Nicholas ; schultz,Judith M.

Report Date : 01 JUL 1966

Pagination or Media Count : 18

Abstract : Simple procedures are described which can give valuable information on the extent of peroxy and other oxidation products in I.V. fat emulsions. It can thus serve as a guide in animal and clinical testing of emulsions. The pH of stored I.V. emulsions continues to show a downward trend. Thin Layer Chromatography (TLC) analysis done after each pH analysis shows no drastic changes in lipid composition. Chemical studies with chromatography will continue to follow pH changes until emulsions break. The introduction of a densitometric scanning device for standard TLC plates will further the development of methods combining well-established chromatographic, chemical and spectrophotometric procedures for the characterization and quantitation of closely related chemical classes of compounds. The main arguments in favor of scanning procedures combined with TLC are productivity and economy of time and effort. With the exception of GLC, the scanning method appears to be the only procedure in which the speed of quantitative determination will match the speed of the chromatographic method of separation. The method furthermore, has the advantage that trace components of samples can be measured directly without using isolation procedures. This new approach may well prove to be important in biochemical research, particularly with I.V. fat emulsions where a large number of samples require characterization and estimations. (Author)

Descriptors :   (*PARENTERAL INFUSIONS, LIPIDS), (*FATS, COLLOIDS), (*LIPIDS, *PEROXIDES), TEST METHODS, THIN LAYER CHROMATOGRAPHY, POLYMERS, PH FACTOR, STORAGE, FREEZING, DENSIMETERS

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE