Accession Number : AD0638387

Title :   BIOCHEMICAL PROPERTIES OF INNER EAR FLUIDS: METHODS OF MICROCHEMISTRY AND FLUID WITHDRAWAL.

Descriptive Note : Final rept. May 61-Apr 64.

Corporate Author : SOLANO LABS BERKELEY CALIF

Personal Author(s) : Ulrich ,Marion T. ; Mundle,J. R. , Jr. ; Margen,Sheldon

Report Date : MAR 1966

Pagination or Media Count : 32

Abstract : Biochemical assay of fluids of the inner ear is pertinent to understanding mechanisms of electrical current generation by living tissue and could elucidate the physiology of acoustic trauma. Quantity of fluid available is limited to a few microliters per sample. A method is presented for collecting fluids from the cochlea while simultaneously measuring DC electrical potentials to identify endolymph. Ultra-micro biochemical and enzyme analyses were developed with adaptations in equipment and procedure. Techniques for assay of sodium, potassium, chloride, sugar, protein, adenosine triphosphate, hexokinase, phosphocreatine, diphosphopyridine nucleotide, lactic dehydrogenase, malic dehydrogenase and adenosine diphosphate in sample volumes of 1-2 microliters are outlined. Data on sodium, potassium, chloride and protein content of endolymph and perilymph agreed with results obtained by other investigators. Sugar averaged 117 mg/100 ml in perilymph and 183 mg/100 ml in endolymph. The enzyme compounds, investigated only in perilymph, were not detectable by these methods on 1 microliters samples. (Author)

Descriptors :   (*EAR, *TISSUE FLUIDS), COLLECTING METHODS, CHEMICAL ANALYSIS, ELECTROPHYSIOLOGY, LYMPH, HEARING, DEAFNESS, ENZYMES, GUINEA PIGS

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE