Accession Number : AD0638638

Title :   THE RELATION BETWEEN AMINO ACID AND GLUCOSE METABOLISM IN DEVELOPING BRAIN.

Descriptive Note : Final rept., May 65-May 66.

Corporate Author : KEIO UNIV TOKYO (JAPAN) SCHOOL OF MEDICINE

Personal Author(s) : Takagaki,Genkichiro

Report Date : AUG 1966

Pagination or Media Count : 18

Abstract : Glutamic acid, the concentration of which in brain is higher than that of any other amino acid, increases the aerobic glucose metabolism of brain tissue in vitro. This effect was concluded to be due to the accumulated potassium ions in brain tissue, the accumulation of which was induced by glutamic acid. Ouabain inhibited the effect of glutamate on glycolysis as well as the accumulation of potassium ions. Aspartic acid inhibits the effect of glutamate on glycolysis but not the accumulation of potassium ions. Aspartic acid which was accumulated in brain tissue in vitro did not show any efflux almost at all, although glutamic acid showed significantly. Aspartic acid might be thought to be firmly attached to cellular components of brain tissue and thus to inhibit the uptake of exchangeable glutamate and potassium ions. Effect of high concentration of potassium ions on the aerobic glucose metabolism of brain tissue in vitro could be fully explained by its effect on pyruvate kinase activity in the glycolytic pathway. Pyruvate concentration after the incubation of brain slices in the addition of phosphoenolpyruvate as substrate was ten times higher in the presence of high concentration of potassium ions in the medium than in the absence. (Author)

Descriptors :   (*AMINO ACIDS, *BRAIN), (*GLUCOSE, *METABOLISM), CHROMATOGRAPHIC ANALYSIS, ENZYMES, TRACER STUDIES, PYRUVIC ACID, GLUTAMIC ACID, CARDIAC GLYCOSIDES, GLYCOLYSIS, ASPARTIC ACID, INHIBITION, POTASSIUM, IN VITRO ANALYSIS, GUINEA PIGS, JAPAN

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE