Accession Number : AD0641910

Title :   AN ABSORPTION SPECTROPHOTOMETER CELL FOR USE AT HIGH PRESSURES AND ITS APPLICATION TO AN ENZYMIC REACTION.

Descriptive Note : Research rept.,

Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD

Personal Author(s) : Steiner,Robert F.

Report Date : 07 NOV 1966

Pagination or Media Count : 16

Abstract : A cell designed for absorbancy measurements at high pressures has been constructed and used for a study of the pressure dependence of a thrombincatalyzed hydrolysis. Thrombin is not inactivated at pressures up to 20,000 psi. The rate of hydrolysis of a synthetic substrate (toluene sulfonyl arginine methyl ester) increases appreciably with pressure. (Author)

Descriptors :   (*SPECTROPHOTOMETERS, CONTAINERS), (*ENZYMES, HIGH PRESSURE), (*ULTRAVIOLET SPECTROSCOPY, INSTRUMENTATION), ABSORPTION SPECTRA, OPTICAL EQUIPMENT COMPONENTS, HYDROLYSIS, CATALYSIS, THROMBIN, REACTION KINETICS, DEEP SUBMERGENCE

Subject Categories : Biochemistry
      Medical Facilities, Equipment and Supplies
      Test Facilities, Equipment and Methods

Distribution Statement : APPROVED FOR PUBLIC RELEASE