Accession Number : AD0643192

Title :   REGULATION OF GLYCOLYSIS IN MOUSE BRAIN IN SITU DURING CONVULSIONS.

Descriptive Note : Technical rept. Jun 65-Jun 66,

Corporate Author : EDGEWOOD ARSENAL MD

Personal Author(s) : Sacktor,Bertram ; Wilson,John E. ; Tiekert,Carvel G.

Report Date : NOV 1966

Pagination or Media Count : 26

Abstract : The concentrations of metabolites, including glucose, glycogen, glycolytic intermediates, adenine nucleotides, phosphates, amino acids, and citrate, in mouse brain in situ were measured from 15 to 180 sec after exposure of mice to the convulsant, 'Indoklon' (bis(2,2,2-trifluoroethyl) ether). An increase in glycolytic flux of at least three times the basal rate was estimated for the convulsing brain. During the period of hyperactivity, new, steady-state levels were established for several metabolites. In the transition, the concentrations of glucose and hexose monophosphates in the brain decreased. Coincident with these decreases, the concentration of fructose-1, 6-diphosphate increased rapidly, but transiently. The concentration of cerebral glycogen did not change during the first 30 sec, decreased about 20% during the next minute, and then remained constant. Lactate accumulated throughout the period of convulsion. At the initiation of convulsion, the concentration of creatine-P precipitously fell to half its original value. The decrease in ATP was relatively small. Correspondingly, the concentration of ADP, AMP, and Pi in the brain increased rapidly with the induction of hyperactivity. The concentrations of citrate, glutamate, and aspartate did not change significantly. The level of proline was lowered during the convulsion. With the induction of convulsions concomitant with the increased rate of glycolysis, the enzymes, phosphofructokinase and hexokinase, were coordinately activated. (Author)

Descriptors :   (*CONVULSIVE DISORDERS, GLYCOLYSIS), BRAIN, SACCHARIDE PHOSPHATES, ADENOSINE PHOSPHATES, ENZYMES, GLYCOGEN, LACTATES, MICE

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE