Accession Number : AD0644709

Title :   EFFECTS OF RADIO-FREQUENCY IRRADIATIONS ON THE ENZYMES OF BEEF MUSCLE TISSUE.

Descriptive Note : Progress rept. no. 1, 13 Apr-13 Aug 64.

Corporate Author : MELPAR INC FALLS CHURCH VA

Report Date : 13 AUG 1964

Pagination or Media Count : 41

Abstract : The natural proteolytic enzyme of beef muscle has been extracted and purified, using procedures analogous to those described in the literature for isolation of proteolytic activity from rabbit muscle. The activity of the enzyme has been differentiated from that of pepsin and trypsin, and some of its characteristics have been established. Appropriate assay procedures have been developed based on the hydrolyses of urea denatured hemoglobin as measured by increase in optical absorbance at 280 millimicrons. Active purified preparations of the proteolytic enzyme have been subjected to radio-frequency irradiation over a limited range in beginning to determine the correct frequency at which the enzyme is most susceptible to attack in vitro. Differences between the activity of irradiated and nonirradiated aliquots of the enzyme are determined by appropriate statistical procedures. (Author)

Descriptors :   (*IRRADIATED FOOD, BEEF), (*BEEF, PEPTIDE HYDROLASES), (*PEPTIDE HYDROLASES, RADIOBIOLOGY), (*ELECTROMAGNETIC RADIATION, RADIOBIOLOGY), PH FACTOR, TEMPERATURE, PURIFICATION, FREEZE DRYING, ENZYMES, RADIOFREQUENCY, STATISTICAL ANALYSIS

Subject Categories : Radiobiology
      Food, Food Service and Nutrition

Distribution Statement : APPROVED FOR PUBLIC RELEASE