Accession Number : AD0645187

Title :   IODOACETATE INHIBITION OF GALACTOSIDE TRANSPORT,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Spoerl,Edward ; Pfeiffer,W. S.

Report Date : 03 NOV 1966

Pagination or Media Count : 13

Abstract : Iodoacetic acid (IAA) was shown to inhibit the in vivo hydrolysis of o-nitro-phenyl-beta-D-galactopyranoside by E. coli K12. Because other work has shown the rate of this hydrolysis to be minimally energy dependent, the IAA effect evidently occurs directly upon the transport system and not upon energy requirements of the system. Exit from the cell of a non-metabolized galactoside was shown to be speeded by inhibitors of energy metabolism, but slowed by IAA, thus, substantiating a direct effect of IAA upon transport. Planned studies of IAA effects upon other transport systems and of possible differential effects upon a permease and a carrier to substantiate the existence of such components were not completed. (Author)

Descriptors :   (*ENZYME INHIBITORS, *ACETIC ACID), TRANSPORT PROPERTIES, INHIBITION, CELLS(BIOLOGY), ESCHERICHIA COLI, HYDROLYSIS

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE