Accession Number : AD0648127

Title :   THE EFFECT OF OXYGEN ON DOG PLASMA SULFHYDRYL GROUPS IN VITRO.

Descriptive Note : Rept. for Jul 65-Mar 66,

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Bartek,Methodius J.

Report Date : JAN 1967

Pagination or Media Count : 12

Abstract : A method is described in which biologic fluids are continuously exposed to a test atmosphere, and sampling is achieved without disruption of the atmosphere. Samples are withdrawn at intervals and analyzed for sulfhydryl content by amperometric titration with the rotating platinum electrode using AgNO3 as the titrant. Inasmuch as this project was undertaken to study the effect of oxygen on sulfhydryl-containing enzymes in vitro, the system was first evaluated using dog plasma as the source of protein. The decrease in dog plasma sulfhydryl content over a 4-hour exposure was: 9.3% for nitrogen, 24.7% for air, and 39.2% for oxygen. (Author)

Descriptors :   (*BLOOD CHEMISTRY, *THIOLS), (*OXYGEN, TOXICITY), STRESS(PHYSIOLOGY), ENZYMES, BLOOD PLASMA, DOGS, IN VITRO ANALYSIS, NITROGEN, AIR

Subject Categories : Biochemistry
      Stress Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE