Accession Number : AD0650863

Title :   RESISTANCE OF LONG-LIVED LYMPHOCYTES AND PLASMA CELLS IN RAT LYMPH NODES TO TREATMENT WITH PREDNISONE, CYCLOPHOSPHAMIDE, 6 MERCAPTOPURINE, AND ACTINOMYCIN D,

Corporate Author : NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s) : Miller,J. J. , III ; Cole,L. J.

Report Date : 20 FEB 1967

Pagination or Media Count : 28

Abstract : The cells of the popliteal lymph nodes of rats were labeled for four days after a secondary immunological stimulus. Thirty-one days after the last dose of tritiated thymidine, groups of rats were started on courses of daily, intraperitoneal injections of prednisone, cyclophosphamide, 6-mercaptopurine, or actinomycin D. The initially low doses of these agents were doubled on successive weeks until either lymphoid hypoplasia or death occurred. Rats from each group were killed weekly, and the percentages of persisting, labeled small lymphocytes in the popliteal nodes were determined. Sections of these nodes were examined for persisting, labeled plasma cells. The percent of lymphocytes labeled increased while the total number of lymphocytes decreased during treatment with prednisone and cyclophosphamide. Prednisone decreased the numbers of long-lived plasma cells, but these cells were preferentially resistant to cyclophosphamide. Neither 6-mercaptopurine nor actinomycin D had an appreciable effect on lymphoid tissues histologically nor on the proportions of labeled, long-lived lymphocytes and plasma cells before causing the deaths of the rats receiving them. These results indicate that long-lived lymphocytes and plasma cells survive treatment with the immunolytic drugs studied, and that long-lived lymphocytes are specifically resistant to prednisone and cyclophosphamide. It is believed that these results have an application to the attempts to find drugs useful in the treatment of immunologic rejections of organ transplants and for therapy of autoimmune diseases. (Author)

Descriptors :   (*TRANSPLANTATION, IMMUNOLOGY), (*CHEMOTHERAPEUTIC AGENTS, IMMUNOLOGY), (*ANTIGEN ANTIBODY REACTIONS, RESISTANCE(BIOLOGY)), (*LYMPHOCYTES, RADIATION EFFECTS), THYMIDINES, TRITIATED COMPOUNDS, DOSAGE, LABELED SUBSTANCES, HISTOLOGY, LYMPHATIC SYSTEM, IMMUNITY

Subject Categories : Medicine and Medical Research
      Radiobiology
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE