Accession Number : AD0652245

Title :   AUTOMATION OF A TECHNIQUE FOR DETERMINING BACTERIAL SENSITIVITY TO ANTIBIOTICS, VOLUME I.

Descriptive Note : Final rept., 15 Oct 66-15 Mar 67,

Corporate Author : SPACE-GENERAL CORP EL MONTE CALIF

Personal Author(s) : Carleton,L. T. ; Miller,C. M. ; Reese,G.

Report Date : MAR 1967

Pagination or Media Count : 102

Abstract : Several hundred bacterial growth curves were generated by the Lindberg-Reese automated instrument for testing sensitivity to antibiotics. The instrument monitors 24-hour growth in liquid media in the presence of graded levels of antibiotics. The curves, computer-plotted from instrument output tapes, reveal numerous details of growth and inhibition for E. coli, Streptococcus faecalis, Staphylococcus aureus, Proteus americanus and Vibrio metschnikovii. Intercomparison showed good reproducibility of fine details, sensitive and characteristic responses to presence of antibiotics, and small but significant effects of inoculum levels. As expected, the nature of the growth medium strongly affected the curve shapes. Further accumulation and analysis of such data should permit prediction of 24-hour minimum inhibitory concentrations from readings after only eight hours. Concepts of instrumentation were developed for the breadboard to be designed and constructed in the next phase and delivered at the end of the program. As compared with (1) the existent research instrument, and (2) the more advanced, originally proposed breadboard, the following principal improvements were visualized: (a) a means of operating continuously, while introducing and removing samples at will; (b) additon of a reference light source for cancelling photomultiplier drift; and (c) a new printout of growth data which is clear and concise and which may eliminate the need for computer processing. (Author)

Descriptors :   (*ANTIBIOTICS, RESISTANCE(BIOLOGY)), BACTERIA, SENSITIVITY, CHEMOTHERAPEUTIC AGENTS, TEST METHODS, GROWTH(PHYSIOLOGY), AUTOMATION, ESCHERICHIA COLI, STREPTOCOCCUS, VIBRIO, STAPHYLOCOCCUS, PROTEUS, PENICILLINS, CHLORAMPHENICOL, STREPTOMYCINS, LABORATORY EQUIPMENT

Subject Categories : Medical Facilities, Equipment and Supplies
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE