Accession Number : AD0652607
Title : CHROMATOGRAPHIC ISOLATION OF SOME CELLULOSE OLIGOSACCHARIDES.
Descriptive Note : Research note,
Corporate Author : FOREST PRODUCTS LAB MADISON WIS
Personal Author(s) : Feather,Milton S. ; Harris,John F.
Report Date : MAY 1967
Pagination or Media Count : 8
Abstract : The preparation of cellotriose and cellotetraose from cellulose is described. The compounds were obtained as the pure alpha acetates by column chromatographic separation of the methanol soluble portion of a partial acetolyzate of cellulose. Application of 15 grams of the acetolyzate to a 6.5- by 50-centimeter silica gel column, followed by elution with benzene-ethyl acetate (1:1) gave 2.1 grams of alpha cellotriose hendecaacetate and 2.0 grams of alpha cellotetraose tetradecaacetate. Elution from a free-flowing column required 12 liters of irrigant and took about 4 days. Chromatographically pure crystalline cellotriose and cellotetraose were obtained by standard deacetylation procedures. (Author)
Descriptors : (*CELLULOSE, CHROMATOGRAPHIC ANALYSIS), (*POLYSACCHARIDES, CHROMATOGRAPHIC ANALYSIS), CARBOHYDRATES, ACETATES, SEPARATION, TRISACCHARIDES, SOLVENTS, HYDROLYSIS
Subject Categories : Biochemistry
Wood, Paper and Related Forestry Products
Distribution Statement : APPROVED FOR PUBLIC RELEASE