Accession Number : AD0652607

Title :   CHROMATOGRAPHIC ISOLATION OF SOME CELLULOSE OLIGOSACCHARIDES.

Descriptive Note : Research note,

Corporate Author : FOREST PRODUCTS LAB MADISON WIS

Personal Author(s) : Feather,Milton S. ; Harris,John F.

Report Date : MAY 1967

Pagination or Media Count : 8

Abstract : The preparation of cellotriose and cellotetraose from cellulose is described. The compounds were obtained as the pure alpha acetates by column chromatographic separation of the methanol soluble portion of a partial acetolyzate of cellulose. Application of 15 grams of the acetolyzate to a 6.5- by 50-centimeter silica gel column, followed by elution with benzene-ethyl acetate (1:1) gave 2.1 grams of alpha cellotriose hendecaacetate and 2.0 grams of alpha cellotetraose tetradecaacetate. Elution from a free-flowing column required 12 liters of irrigant and took about 4 days. Chromatographically pure crystalline cellotriose and cellotetraose were obtained by standard deacetylation procedures. (Author)

Descriptors :   (*CELLULOSE, CHROMATOGRAPHIC ANALYSIS), (*POLYSACCHARIDES, CHROMATOGRAPHIC ANALYSIS), CARBOHYDRATES, ACETATES, SEPARATION, TRISACCHARIDES, SOLVENTS, HYDROLYSIS

Subject Categories : Biochemistry
      Organic Chemistry
      Wood, Paper and Related Forestry Products

Distribution Statement : APPROVED FOR PUBLIC RELEASE