Accession Number : AD0654700

Title :   ASSOCIATION OF NEUTRAL DEOXYRIBONUCLEASE WITH CHROMATIN ISOLATED FROM MAMMALIAN CELLS,

Corporate Author : NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s) : Swingle,Karl F. ; Cole,Leonard J. ; Bailey,J. Stanley

Report Date : 29 MAY 1967

Pagination or Media Count : 24

Abstract : Nuclei prepared from spleen cells by a variety of published procedures retained a deoxyribonuclease (DNase) which caused a partial degradation of the nuclear DNA when the nuclei were incubated in the presence of divalent cations. Well-washed chromatin prepared according to Paul and Gilmour (8) from disrupted nuclei of either spleen or liver retained the DNase, but the soluble nucleoprotein of Zubay and Doty was free of it. The latter preparation was also resistant to attack by added DNase I. The nuclear DNase was shown to be of the DNase I type, which produces 3'-hydroxylterminated fragments of DNA. X-irradiation of washed nuclei in vitro did not alter their susceptibility to degradation by their endogenous DNase. Chromatin from 'free' thymocytes did not contain endogenous DNase I activity. (Author)

Descriptors :   (*RIBONUCLEASE, NUCLEOPROTEINS), (*DEOXYRIBONUCLEIC ACIDS, *RADIATION EFFECTS), X RAYS, THYMUS, SPLEEN, CELLS(BIOLOGY), NUCLEI(BIOLOGY), DEGRADATION, LIVER, IN VITRO ANALYSIS

Subject Categories : Biochemistry
      Radiobiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE