Accession Number : AD0657859

Title :   USE OF GERMFREE ANIMALS IN THE STUDY OF DENTAL CARIES.

Descriptive Note : Final technical rept. 1 Aug 62-31 Jul 65,

Corporate Author : CHICAGO UNIV ILL ZOLLER MEMORIAL DENTAL CLINIC

Personal Author(s) : Orland,Frank J. ; Wagner,Morris ; Carbonell,Virginia M.

Report Date : 07 SEP 1967

Pagination or Media Count : 5

Abstract : Over the years, it has been possible to demonstrate that in the complete absence of all bacteria, germfree rats could not develop dental decay even though ingesting a caries-inducing diet for as long as 150 days when the early experiments were terminated. Later, in the presence of an enterococcus (Streptococcus sp) isolated from a rat in the external environment and with dental caries, otherwise germfree rats developed caries, though a longer time period was involved. Both in one early group of rats, as well as in several later groups of otherwise germfree rats inoculated with a lactobacillus strain, originally isolated from a human mouth with caries, dental caries did occur in some rats' molar teeth, but especially so in subsequent generations of rats living in such a lactobacillus environment. Inoculated leptotrichia-like filamentous bacteria were difficult to establish and failed to induce caries in otherwise germfree (gnotobiotic) rats. Finally, in recently-observed animals maintained their life span on caries-inducing diets, no dental decay could be detected, thus conclusively demonstrating the etiological relationship of bacteria and dental caries in this kind of unique study. (Author)

Descriptors :   (*TEETH, DISEASES), (*GERM FREE ANIMALS, TEETH), DENTISTRY, DIET, STREPTOCOCCUS, LACTOBACILLUS, ETIOLOGY, RATS

Subject Categories : Animal Husbandry and Veterinary Medicine
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE