Accession Number : AD0658850

Title :   THE EFFECT OF SOME PROTECTIVE SUBSTANCES ON THE PROCESS OF FREEZING AND THAWING OF CELLULAR ELEMENTS IN THE BLOOD.

Descriptive Note : Figs. refs. omitted,

Corporate Author : NAVAL MEDICAL SCHOOL BETHESDA MD TRANSLATION SERVICE

Personal Author(s) : Teodorovich,V. I. ; Kozlov,G. A.

Report Date : 11 AUG 1967

Pagination or Media Count : 16

Abstract : Damage to blood cells in blood taken with the usual glucose-citrate solution with out the addition of protective substances, occurs during the process of freezing and thawing, apparently, occurs essentially during the stage of thawing. Ethyl alcohol, used as a protective substance, in a complex glucose-citrate solution, protects to a certain degree, the blood cells during the process of freezing and thawing in spite of intensive re-crystallization, even at relatively low temperature. In the concentrated solution, containing two protective substances (ethyl alcohol and glycerine), the process of recrystallization is weak. At temperatures to -50C a considerable portion of the solution remains non-crystallized and fills up the canals between the crystals in which there are present some mobile cells. (Author)

Descriptors :   (*BLOOD CELLS, FREEZING), STORAGE, HEATING, PROTECTION, ADDITIVES, ETHANOLS, GLUCOSE, CITRATES, GLYCEROLS, CRYSTALLIZATION, PRESERVATION, BLOOD

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE