Accession Number : AD0659476

Title :   THE MIXED LEUCOCYTE REACTION APPLIED TO INBRED MOUSE STRAINS,

Corporate Author : NAVAL RADIOLOGICAL DEFENSE LAB SAN FRANCISCO CALIF

Personal Author(s) : Main,Raymond K. ; Jones,Marjorie J. ; Cole,Leonard J.

Report Date : 16 AUG 1967

Pagination or Media Count : 30

Abstract : The mixed leucocyte reaction, (MLR), has been applied successfully to peripheral blood leucocytes of the mouse. Before harvest, the leucocytes were mobilized into the peripheral blood by a single intravenous injection of the mice with pertussis vaccine. Mixtures, (50-50), of C57BL/6 and DBA/2 mouse leucocytes were cultured in medium containing low amounts of mouse plasma and supplemented with foetal bovine serum. DNA-synthetic activities at selected times were determined by liquid scintillation counting following pulse labeling of the cell populations with 3H-TdR. DNA synthesis in the mixed cultures attained a maximum value at the 5th to 7th day (7,500 cpm), as contrasted with maximum control values of 200-1000 cpm). Considerable DNA synthesis (1000-2000 cpm), also was observed at zero time, and then declined to low levels (200-300 cpm) at the 18th hour. DNA synthesis did not occur in the mixed leucocyte cultures when the culture medium was supplemented with dog plasma in place of foetal bovine serum, or when the leucocyte cultures were contaminated with appreciable numbers of erythrocytes. (Author)

Descriptors :   (*TRANSPLANTATION, *IMMUNOLOGY), (*LEUKOCYTES, MICE), (*ANTIGEN ANTIBODY REACTIONS, LEUKOCYTES), GENETICS, TISSUE CULTURE, DEOXYRIBONUCLEIC ACIDS, BIOSYNTHESIS, TISSUES(BIOLOGY), CELLS(BIOLOGY), LABELED SUBSTANCES, TRITIATED COMPOUNDS, THYMIDINES, IN VIVO ANALYSIS, CULTURE MEDIA

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE