Accession Number : AD0659720

Title :   LABORATORY CONTROL OF ANTICOAGULATION THERAPY; QUALITY CONTROL IN COAGULATION PROCEDURES IN ONE-STAGE PROTHROMBIN TIME.

Descriptive Note : Final rept.,

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Bairrington,Jarrell D. ; Peterson,Emil W.

Report Date : JUN 1967

Pagination or Media Count : 15

Abstract : The study involved a comparison of 0.1 M sodium oxalate and 3.8% sodium citrate as plasma decalcifying agents for use with the one-stage prothrombin time. Samples of blood were drawn from 10 normal humans and 10 patients on long-term, coumarin-derivative therapy. One-stage prothrombin determinations were made on stoppered and unstoppered samples incubated at 4, 27, and 37C. after 4 hours of incubation. Factor V and Factor VII were added to the specimens after incubation, and a prothrombin determination was made. Factor V activity began decreasing as early as 15 minutes after the specimen was drawn and was rapidly and progressively decreased in samples decalcified with 0.1 M sodium oxalate, resulting in an increase in the prothrombin time. In contrast, 3.8% sodium citrate seemed to stabilize Factor V and is recommended for routine use as the plasma decalcifying agent of choice in the performance of the one-stage prothrombin time. (Author)

Descriptors :   (*ANTICOAGULANTS, THERAPY), (*PROTHROMBIN, CHEMICAL ANALYSIS), BLOOD COAGULATION, SODIUM COMPOUNDS, CITRATES, OXALATES, TEST METHODS, BLOOD CHEMISTRY, ACCURACY, BLOOD PLASMA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE