Accession Number : AD0664761

Title :   INHIBITION BY METHYLPHENIDATE OF TRANSPORT ACROSS THE YEAST CELL MEMBRANE.

Descriptive Note : Final rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Spoerl,Edward ; Doyle,R. J.

Report Date : 08 NOV 1967

Pagination or Media Count : 20

Abstract : Methylphenidate at a concentration of 10 mM inhibited CO2 production and O2 uptake of yeast cells by 75%. This effect, with glucose as a substrate, occurred at pH 7.0, but not at pH 4.5. Kinetic data indicated the reaction was complex, and after binding, methylphenidate could not be readily removed. Glycolysis by cell free extracts was not inhibited at the 10 mM concentration, but was affected at 100 mM. O2 utilization with maltose and ethyl alcohol as substrates also was reduced. Entry into the cells of a number of different carbohydrates and glycine was inhibited to different degrees. The loss of 280 millimicron absorbing materials from suspended cells was reduced and the efflux of sorbose, arabinose and lactose was decreased. Thus, transport into and out of the cells was inhibited, and leakage, or permeability, was reduced. A general reaction was a cell membrane constituent or constituents is indicated. (Author)

Descriptors :   (*YEASTS, METABOLISM), CNS STIMULANTS, TRANSPORT PROPERTIES, PERMEABILITY, MEMBRANES(BIOLOGY), CELLS(BIOLOGY), GLYCOLYSIS, INHIBITION, CARBON DIOXIDE, OXYGEN CONSUMPTION

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE