Accession Number : AD0666422

Title :   AN AUTOMATED, CLOSED SYSTEM FOR GAS ANALYSIS: ASSAY OF CARBON MONOXIDE IN BLOOD.

Descriptive Note : Rept. for Dec 65-Jul 67,

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Wease,Dorothy F. ; Stansell,Marion J. ; Espinosa,Eli S.

Report Date : DEC 1967

Pagination or Media Count : 19

Abstract : A closed, automated system for the determination of carbon monoxide is described. The technic was found applicable to the assay of large numbers of blood samples with carbon monoxide content ranging from background levels up to lethal concentrations. The limits of assay for this method are 2 to 10 micromoles. CO per 100 ml. of whole blood, which is equivalent to 0.5 to 2.5 p.p.m. (w/w). Samples containing higher concentrations are appropriately diluted with degassed physiologic saline. Analysis of the optical density per micromole % CO obtained over several months using different bloods with hemoglobin concentrations ranging from 11.7 to 13.0 gm. %, showed a maximum deviation from the overall average of less than 0.005 OD. Whole blood recovery data ranged from 97% to 103% with an average value of 99.5%. The basic technic offers an approach to the quantitative assay of a wide variety of body and environmental gases, both those that are toxic and those that are vitally essential. (Author)

Descriptors :   (*BLOOD CHEMISTRY, CARBON MONOXIDE), (*CARBON MONOXIDE, BLOOD ANALYSIS), GAS ANALYSIS, TEST METHODS, QUANTITATIVE ANALYSIS, MICROANALYSIS, BLOOD ANALYSIS, AIR POLLUTION, CHEMICAL INDICATORS

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE