Accession Number : AD0666904

Title :   IN VITRO DETERMINATION OF PLASMA INSULIN USING 125I AND LIQUID SCINTILLATION SPECTROMETRY.

Descriptive Note : Rept. for Aug-Oct 67,

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Logsdon,Donald F. , Jr. ; Green,James F. ; Langford,Orville L.

Report Date : JAN 1968

Pagination or Media Count : 9

Abstract : A procedure for the immunoassay of insulin levels using insulin 125I as a tracer was modified to allow the samples to be measured by means of a liquid scintillation spectrometer. The optimal instrument settings and scintillation fluid system were determined. The absolute efficiency was found to be 21.4%. The components making up the sample were individually tested and found to have no effect on the count rate. The percent change in activity with increasing amounts of added cold insulin (over the range 0 to 500 micro U.) was determined using both the liquid scintillation system and the gamma spectrometer. No significant difference was found between the two methods in terms of accuracy or ease of accomplishing the procedure. The two systems are considered interchangeable for counting insulin 125I samples. (Author)

Descriptors :   (*INSULIN, TRACER STUDIES), SCINTILLATION COUNTERS, SPECTROMETERS, EFFICIENCY, ACCURACY, COUNTING METHODS, IN VITRO ANALYSIS, BLOOD CHEMISTRY, BLOOD PLASMA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE