Accession Number : AD0666908

Title :   DETERMINING INSULIN LEVELS USING INSULIN I 125 AND INSULIN-BINDING REAGENT.

Descriptive Note : Rept. for Apr-Aug 67,

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TEX

Personal Author(s) : Green,James F.

Report Date : DEC 1967

Pagination or Media Count : 13

Abstract : A procedure for the immunoassay of insulin levels using insulin I 125 as a tracer was evaluated to study parameters of reproducibility, accuracy of values, sensitivity with changes in fasting times, temperature effects, and recovery. On the basis of the evaluation, the method is considered to be appropriate as a routine diagnostic test for fasting insulin levels. The usefulness of this procedure for measurement of insulin levels through the course of a standard glucose test has yet to be evaluated. Future study should involve evaluation of the concept of free and bound insulin, determining which insulin this system measures, and the relationship of free to bound insulin during both fasting and glucose tolerance conditions in normal individuals. (Author)

Descriptors :   (*INSULIN, TRACER STUDIES), DIAGNOSIS(MEDICINE), GLUCOSE, TOXIC TOLERANCES, RADIOACTIVE ISOTOPES, SENSITIVITY, CARBOHYDRATES, METABOLISM, IMMUNOLOGY

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE