Accession Number : AD0672105

Title :   THE USE OF DARK-FIELD ILLUMINATION IN THE ELECTRON MICROSCOPE FOR MICROORGANISM STRUCTURE STUDY,

Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s) : Petrova,G. P. ; Ponomarev,K. A.

Report Date : 01 JUL 1968

Pagination or Media Count : 6

Abstract : With the assistance of the accessories that were installed in the electron microscope EM-3 it is possible to receive a dark-field illumination of a given area of an object and to change it to a bright field of the same area in short period of time. The dark-field method in electron microscopy is perspective for investigation of the internal structures of bacterial cells. In the dark field the difference in the character of the electron diffusion by the bodies of the gram-positive and gram-negative bacteria is clearly shown. It is expressed in that the gram-positive and gram-negative forms appear dark in the bright field, whereas in the dark field the gram-positive forms are dark and the gram-negative bright. A combination of the methods of investigating an object in the dark and bright field gives a more complete representation of the object's structure than either of them can give separately. (Author)

Descriptors :   (*ELECTRON MICROSCOPY, MICROORGANISMS), CELLS(BIOLOGY), VIRUSES, BACTERIA, HISTOLOGICAL TECHNIQUES, CELL STRUCTURE, ELECTRON MICROSCOPES, SPORES, USSR

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE