Accession Number : AD0673019

Title :   THE GROWTH OF JAPANESE AND WEST NILE ENCEPHALITIS VIRUSES IN TISSUE CULTURES,

Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s) : Fan,Tsi-min

Report Date : 01 JUL 1968

Pagination or Media Count : 8

Abstract : The cytopathogenic effect, which was registered from the first passage, was regularly detected in the cultivation of three strains of the JE virus (Ya-47, R-1, K-2) and one strain of the West Nile encephalitis virus in cultures of monkey-heart cells. Practically identical results were noted in a parallel titration of the virus in tissue cultures and in mice. The specificity of the virus's cytopathogenic effect was proved by means of extinguishing it with a homologous immune serum. In the HeLa cells a specific cytopathogenic effect was registered after six cultural passages of the strain R-1. The cytopathogenic effect was retained in subinoculation. The West Nile encephalitis virus induced a cytopathogenic effect in the HeLa cells from the first passage. The Japanese encephalitis virus (strains Ya-47, R-1, K-2, K-4, and 'Nakayama') and the West Nile encephalitis virus propagated in the cultures of skin-muscle tissue of the human and chick embryos, but they did not induce a cytopathogenic effect. The mean titer and the viruses in an intracerebral inoculation of mice (lg LD50) was 5.6 and 5.0 respectively. (Author)

Descriptors :   (*GROUP B ARBOVIRUSES, TISSUE CULTURE), JAPANESE B ENCEPHALITIS VIRUS, WEST NILE VIRUS, GROWTH(PHYSIOLOGY), IMMUNE SERUMS, DEGRADATION, TISSUE CULTURE CELLS, USSR

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE