Accession Number : AD0674298

Title :   MONOAMINERGIC MECHANISMS IN NERVOUS TISSUE.

Descriptive Note : Final rept. 1 Nov 66-31 Oct 67,

Corporate Author : LUND UNIV (SWEDEN) DEPT OF ZOOLOGY

Personal Author(s) : Dahl,Erik ; Falck,Bengt

Report Date : 15 APR 1968

Pagination or Media Count : 5

Abstract : Selective denervation experiments in combination with the specific histochemical methods for catecholamines and acetylcholinesterase have shown that the feline dilator pupillae is supplied by numerous parasympathetic as well as sympathetic fibers. Probably fibers of both types accompany each other in the strands of the autonomic ground plexus. A technique for simultaneous demonstration of adrenergic and non-adrenergic fibers has been devised. The method rests on the finding that methylene blue, under certain conditions, will preferably stain non-adrenergic fibers. A new method for the cellular localization of histamine has been worked out. It is based on the principle that di-orthophthalaldehyde under dry conditions forms a fluerophore with histamine. The specificity of the method is high. The histamine fluerophore has been characterized in microspectrometric analyses. The mechanism of ventricular fibrillation during induced hypothermia has been studied in further detail. In cat, prenylamine effectively protects against fibrillation, whose the incidence seems to be related to the number of intact adrenergic nerves present in relation to the cardiac muscles. Hypothermia causes a distinct release of transmitter from the cardiac adrenergic nerves. (Author)

Descriptors :   (*AMINES, AUTONOMIC NERVOUS SYSTEM), (*NERVE FIBERS, HISTOLOGICAL TECHNIQUES), ACETYLCHOLINESTERASE, METHYLENE BLUE, BIOLOGICAL STAINS, HISTAMINE, FLUORESCENCE, HYPOTHERMIA, HEART, NEUROMUSCULAR TRANSMISSION, SWEDEN

Subject Categories : Anatomy and Physiology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE