Accession Number : AD0674360

Title :   GEL FILTRATION OF NATIVE AND MODIFIED PIG SERUM LIPOPROTEINS,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOSCIENCES

Personal Author(s) : Kalab,M. ; Martin,W. G.

Report Date : 15 FEB 1968

Pagination or Media Count : 5

Abstract : Total lipoproteins were isolated from pig blood serum and further separated into individual classes of very low-density (VLDL), low-density (LDL) and high-density (HDL) lipoproteins by preparative centrifugation in NaBr solutions. The same classes were obtained by gel filtration of the total lipoproteins on Biogel A 50m (agarose gel) and were eluted in the order VLDL, LDL and HDL. LDL emerged as a single broad assymmetrical peak. Its initial and final portions were enriched in the subclasses LDL1 and LDL2, respectively. Gel filtration separated partially delipidated LDL into three zones. The first to emerge contained large aggregates, the intermediate and final zone (which eluted in the same volume as LDL) contained several ultracentrifugally distinguishable components. Partially delipidated VLDL emerged in about the same volume as HDL. (Author)

Descriptors :   (*LIPOPROTEINS, ELECTROPHORESIS), BLOOD SERUM, SWINE, DENSITY, CENTRIFUGE SEPARATION, DIALYSIS, GELS, ABSORPTION, BROMIDES, CANADA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE