Accession Number : AD0674362

Title :   THE INVOLVEMENT OF CELLULASE AND LAMINARANASE IN THE FORMATION OF PYTHIUM PROTOPLASTS,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA SASKATOON (SASKATCHEWAN) PRAIRIE REGIONAL LAB

Personal Author(s) : Eveleigh,D. E. ; Sietsma,J. H. ; Haskins,R. H.

Report Date : 27 NOV 1967

Pagination or Media Count : 9

Abstract : Five inducible streptomycete lytic enzyme complexes were equally capable of producing protoplasts from a Pythium sp. strain PRL 2142, when compared on a quantitative basis. Both cellulase and laminaranase from various microbial sources were required to produce the protoplasts under standard conditions. Protease was not required; lipase shortened the survival time of protoplasts. It was concluded from an analysis of the products of enzymic hydrolyses that Pythium sp. PRL 2142 cell wall contained cellulose, beta-D-(1--3) glucan, and a beta-D-(1--3) glucan possessing D-glucose units attached by beta-(1--6)-linkages. (Author)

Descriptors :   (*FUNGI, *ENZYMES), CELLS(BIOLOGY), CELL WALL, DECOMPOSITION, PEPTIDE HYDROLASES, CELLULOSE, MOLECULAR STRUCTURE, HYDROLYSIS, CANADA

Subject Categories : Biochemistry
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE