Accession Number : AD0676104

Title :   ELECTRON MICROSCOPIC LOCALIZATION OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE ACTIVITY.

Descriptive Note : Technical rept. Jan-Jun 67,

Corporate Author : EDGEWOOD ARSENAL MD

Personal Author(s) : Grossman,I. William ; Heitkamp,Dale H.

Report Date : JUL 1968

Pagination or Media Count : 20

Abstract : Magnesium-dependent adenosine triphosphatase (ATPase) activity of unfixed, isolated, intact skeletal muscle mitochondria, frozen-thawed mitochondria, and digitonin-disrupted submitochondrial particles was localized ultrastructurally after incubation in a Wachstein-Meisel reaction medium and correlated with biochemical ATPase activity. The reaction precipitate was localized within the matrix of intact and partially disrupted mitochondria and between membranous fragments in the submitochondrial particles. These findings are consistent with an ATPase activity residing within the headpieces of the mitochondrial cristae with deposition of reaction product within the adjacent matrix. The effects of various activators, inhibitors, nucleotides, and disruptive procedures on this localization are described. (Author)

Descriptors :   (*MITOCHONDRIA, ENZYMES), ELECTRON MICROSCOPY, CELL STRUCTURE, PHOSPHORIC MONOESTER HYDROLASES, MEMBRANES(BIOLOGY), ENZYME INHIBITORS, NUCLEOTIDES, ADENOSINE PHOSPHATES, MUSCLES

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE