Accession Number : AD0677189

Title :   RAPID IDENTIFICATION OF MICROORGANISMS BY CONTINUOUS PARTICLE ELECTROPHORESIS.

Descriptive Note : Final rept. 15 Apr-15 Nov 67,

Corporate Author : BECKMAN INSTRUMENTS INC FULLERTON CALIF ADVANCED TECHNOLOGY OPERATIONS

Personal Author(s) : Huebner,Victor R.

Report Date : AUG 1968

Pagination or Media Count : 24

Abstract : The feasibility of using continuous particle electrophoresis for fractionating and identifying certain specified biologic organisms was investigated. To determine the optimum operating conditions for achieving this goal, various procedures were used, including different bacterial handling methods, various pH values of the certain buffer, and various sterilization procedures. It was found that the five microorganisms of interest could be separated from each other by judicious choice of operating conditions. Several technics potentially capable of automatic screening of unknown bacterial suspensions were also studied. These studies used the Beckman optical scanner to detect microorganisms, determine their electrophoretic mobility, and determine the effect of pH gradients on electrophoretic mobility. These studies resulted in a procedure which may be feasible for completely automatic detection and characterization of microorganisms within a mixture, if the prior history of the mixture is known. (Author)

Descriptors :   (*MICROORGANISMS, IDENTIFICATION), (*ELECTROPHORESIS, MICROORGANISMS), MOBILITY, PH FACTOR, OPTICAL SCANNING, ESCHERICHIA COLI, SHIGELLA FLEXNERI, BACILLUS CEREUS, SERRATIA MARCESCENS, BACILLUS SUBTILIS

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE