Accession Number : AD0678186

Title :   PURIFICATION OF CL. PERFRINGENS ANATOXIN BY THE METHOD OF ELECTROPHORETIC CONVECTION,

Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD

Personal Author(s) : Samsonova,V. S. ; Nikitina,V. D. ; Kholchev,N. V. ; Volkova,Z. M.

Report Date : 24 NOV 1967

Pagination or Media Count : 9

Abstract : The isoelectric point of Cl. perfringens anatoxin, purified via precipitation with HCl at pH 3.8-4.0, comprised 4.8-5.0 upon determination on the MMED apparatus (multimembranous electric decantator). Upon optimum conditions of anatoxin purification by the method of electrophoretic convection on the MMED apparatus, a purification coefficient was obtained equaling 1.6-2.1, at 50% preparation yield, and the activity of 10-100 BU per one milligram of protein nitrogen. When ECD-1 (electric convection device) was used, a preparation was obtained with purification coefficient of 2.7-3.6, up to 36% yield, and 7-30 BU/mg of protein nitrogen activity. With four-stage purification of anatoxin Cl. perfringens (acid + alcohol + 2-stage purification with the electroconvection method), a highly purified preparation of Cl. perfringens anatoxin was obtained, with activity up to 1340-1600 BU/mg of protein nitrogen. The method of electrophoretic convection with the use of MMED and ECD-1 apparatus can be employed for additional purification of Cl. perfringens anatoxins, obtained as a result of one-state purification (precipitation with HCl, at pH 3.8-5.0), or two-stage purification (precipitation with HCl with subsequent precipitation with alcohol). (Author)

Descriptors :   (*TOXINS AND ANTITOXINS, PURIFICATION), (*CLOSTRIDIUM PERFRINGENS, TOXINS AND ANTITOXINS), ELECTROPHORESIS, CHEMICAL PRECIPITATION, SEPARATION, USSR

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE