Accession Number : AD0678396

Title :   REPRODUCTION OF THE PHAGES OF BACILLUS SUBTILIS IN A CELL-LESS BACTERIAL MEDIUM,

Corporate Author : FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO

Personal Author(s) : Bulanov,P. A. ; Polkhovskii,V. A.

Report Date : 15 DEC 1967

Pagination or Media Count : 6

Abstract : In June 1964, P. A. Bulanov synthesized the staphylococcal bacteriophage in a medium containing no living cells. Accordingly, the authors pursued further this line of research in bacterial viruses (phages) which are a unique model for investigating the nature of viruses. To this end, they worked to develop a cell-less bacterial medium in which bacteriophages of Bac. subtilis could be cultured. This nutrient medium was prepared from lysozyme-sensitive yet nonlysogenic strain of Bac. subtilis No. 48 taken from soil in Minskaya Oblast. The concentration of the suspension was 1.5 billion microbial cells per cc. The culture was grown on meat-peptone agar and crystalline lysozyme (0.005 g per 100 cc of suspension) was added. Complete lysis of the cells followed in 10-15 min, whereupon the solution was further processed and 0.2 cc of phages of Bac. subtilis was added to the medium. Phage reproduction was determined according to the lytic activity of the phages. The experiments established the fundamental possibility of growing bacteriophage cultures in cell-less systems. (Author)

Descriptors :   (*BACTERIOPHAGES, REPRODUCTION), BACILLUS SUBTILIS, CULTURE MEDIA, MURAMIDASE, GROWTH(PHYSIOLOGY), CONCENTRATION(CHEMISTRY), MORPHOLOGY(BIOLOGY), VIRUSES, USSR

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE