Accession Number : AD0679417

Title :   BIOSYNTHESIS OF MEMBRANE LIPIDS IN HALOBACTERIUM CUTIRUBRUM,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF BIOSCIENCES

Personal Author(s) : Kates,M. ; Wassef,M. K. ; Kushner,D. J.

Report Date : 1968

Pagination or Media Count : 9

Abstract : Incorporation of C14 into the lipids of the obligate halophile, Halobacterium cutirubrum grown in the presence of C14-labelled acetate, malonate, mevalonate or glycerol was investigated. Mevalonate showed the highest incorporation (8% of the amount supplied), acetate and glycerol were each incorporated to the extent of 2%, and only traces of C14 were incorporated with malonate as precursor. Incorporation with the latter precursor is probably due to prior breakdown of the malonate to acetate. With each of the precursors, extensive labelling of the phytanyl chains was observed, but only minor labelling of the water-soluble moieties, except for (C14) glycerol, with which a considerable proportion of C14 was found in the phosphate ester-sugar fraction. Only traces of C14-labelled fatty acids were detected in the lipids labelled with acetate. It is concluded that the major biosynthetic route for the lipid chains is the mevalonate pathway for synthesis of isoprenoids; the malonyl-CoA pathway for synthesis of fatty acids appears to be operating at a very low level of activity. (Author)

Descriptors :   (*PSEUDOMONADACEAE, *LIPIDS), BIOSYNTHESIS, LABELED SUBSTANCES, ACETATES, GLYCEROLS, THIN LAYER CHROMATOGRAPHY, CANADA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE