Accession Number : AD0681213

Title :   STUDIES ON POLYMERIC STRUCTURE OF HEMOGLOBIN AND ITS RELATION TO THE FUNCTION.

Descriptive Note : Rept. no 2 (Final), Oct 67-Oct 68,

Corporate Author : NARA MEDICAL COLL KASHIHARA CITY (JAPAN) DEPT OF PHYSIOLOGY

Personal Author(s) : Enoki,Yasunori

Report Date : OCT 1968

Pagination or Media Count : 23

Abstract : A simple and high yield procedure for obtaining the gamma F subunit in a physiologically active form was developed. The isolated gamma F subunits were identified by starch gel electrophoresis, peptide mapping and the characteristic ultraviolet absorption spectrum. The number of the reactive SH group was estimated as one per chain. The oxygen equilibrium was characterized by an increased affinity toward oxygen, and absence of heme-heme interaction and the Bohr effect. The oxygen affinity was found to be lower than that of the beta A subunits. All these findings are essentially the same as those for other single subunit hemoglobins. (Author)

Descriptors :   (*HEMOGLOBIN, MOLECULAR STRUCTURE), MOLECULAR ASSOCIATION, MOLECULAR WEIGHT, ELECTROPHORESIS, OXYGEN, EQUILIBRIUM(PHYSIOLOGY), DISTILLATION, BLOOD, DIALYSIS, BLOOD CHEMISTRY, POLYMERS, JAPAN

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE