Accession Number : AD0681898

Title :   IN VIVO AND IN VITRO STUDIES ON BME HEMOGLOBIN.

Descriptive Note : Progress rept.,

Corporate Author : ARMY MEDICAL RESEARCH LAB FORT KNOX KY

Personal Author(s) : Bunn,H. Franklin ; Esham,William T.

Report Date : 07 OCT 1968

Pagination or Media Count : 23

Abstract : Hemoglobin treated with the sulfhydryl reagent BME had a prlonged plasma survival in rats, primarily due to a marked reduction in hemoglobinuria. In nephrectomized rats, the disappearance of BME hemoglobin and untreated hemoglobin was equally prolonged. In dogs, the plasma survival of BME hemoglobin was also prolonged. After human hemoglobin was treated with BME, its rate of auto-oxidation under physiologic conditions was reduced by about half. BME hemoglobin had an extremely high affinity for oxygen, rendering it useless for the physiologic transport of oxygen. Untreated hemoglobin solutions mixed with plasma had a much less marked increase in oxygen affinity, although its oxygen dissociation curve was also significantly to the left of whole blood. (Author)

Descriptors :   (*HEMOGLOBIN, IN VIVO ANALYSIS), (*IN VITRO ANALYSIS, HEMOGLOBIN), (*BLOOD PLASMA SUBSTITUTES, TRACER STUDIES), BLOOD PLASMA, EXCISION, KIDNEYS, RATS, DOGS, OXIDATION, OXYGEN, LABELED SUBSTANCES, INJECTIONS(MEDICINE), TABLES(DATA), THIOLS

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE