Accession Number : AD0682090

Title :   EXTRACELLULAR ENZYME SYSTEM UTILIZED BY THE ROT FUNGUS STEREUM SANGUINOLENTUM FOR THE BREAKDOWN OF CELLULOSE: I. STUDIES ON THE ENZYME PRODUCTION. II. PURIFICATION OF THE CELLULASE. III. CHARACTERIZATION OF TWO PURIFIED CELLULASE FRACTIONS,

Corporate Author : SVENSKA TRAFORSKNINGSINSTITUTET STOCKHOLM

Personal Author(s) : Bucht,Bengt ; Eriksson,Karl-Erik ; Pettersson,Bert ; Bjorndal,Hakan

Report Date : 29 AUG 1967

Pagination or Media Count : 19

Abstract : The production of cellulolytic enzymes by the rot fungus Stereum sanguinolentum has been studied on different carbon sources. When powdered cellulose or carboxymethyl cellulose was used as the carbon source, high yields of extracellular cellulolytic enzymes were obtained, but there was no production of these enzymes with cellobiose as the carbon source. The heterogeneities in the protein structure of the enzymes were studied by the isoelectric focusing method. Also, the separation of two cellulase peaks from culture solutions of the rot fungus Stereum sanguinolentum has been demonstrated on a DEAE-Sephadex A-50 column. After dialysis, when the carbohydrate content of both the enzymes decreased to zero, the mixed enzymes gave one homogeneous cellulase peak upon rechromatography on a DEAE-Sephadex A-50 column as well as on column electrophoresis. The results strongly indicate that S. sanguinolentum only excretes one cellulase enzyme in a culture solution with powdered cellulose as the carbon source. (Author)

Descriptors :   (*FUNGI, ENZYMES), (*ENZYMES, PURIFICATION), CULTURE MEDIA, CELLULOSE, ELECTROPHORESIS, CHROMATOGRAPHIC ANALYSIS, SWEDEN

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE