Accession Number : AD0684245

Title :   OPTIMIZATION OF A SYNTHETIC NUTRIENT MEDIUM FOR CULTURING TYPHOID BACILLI. REPORT 1: SELECTION OF THE MOST ESSENTIAL FACTORS OF A NUTRIENT MEDIUM BY THE METHOD OF ACCIDENTAL BALANCE,

Corporate Author : ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER WASHINGTON D C

Personal Author(s) : Shuster,B. Yu. ; Biryukov,V. V.

Report Date : 18 FEB 1969

Pagination or Media Count : 12

Abstract : The Satterthwaite random balance method was employed to select an optimum nutrient medium for culturing typhoid bacteria. The addition of five supplementary nutrients (dl-aspargic and dl-glutamic acids, dl-lysin, dl-methionine and l-cistine) to the Troitskiy-Gekker synthetic medium resulted in a six-fold increase in microbial mass. On the basis of the Satterthwaite method it was determined that the five factors most influencing the intensity of propagation were: l-tryptophane, dl-asparagic acid, glucose, bisubstituted ammonium citrate and nicotinic acid. Dl-lysin was omitted from the medium because of its competitive interaction with nicotinic acid. (Author)

Descriptors :   (*SALMONELLA TYPHOSA, *CULTURE MEDIA), NUTRITION, OPTIMIZATION, AMINO ACIDS, GROWTH(PHYSIOLOGY), USSR

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE