Accession Number : AD0685663

Title :   HOG CHOLERA VIRUS ATTENUATION IN TISSUE CULTURE,

Corporate Author : ARMY FOREIGN SCIENCE AND TECHNOLOGY CENTER WASHINGTON D C

Personal Author(s) : Airapetyan,V. G. ; Khachatryan,A. B. ; Pogosyan,A. A.

Report Date : 10 MAR 1969

Pagination or Media Count : 9

Abstract : Hog cholera virus has been successfully attenuated in tissue culture. After adaptation of the crude virus to cultures of hog embryo kidney cells, it was passaged 30-40 times. Virulence was retained, while the virus became cytopathogenic and only slightly viable. During stabilization by means of 7-fold passaging of cultured virus to a baby pig, the virus established its potential viability. The stabilized virus was repeatedly passaged 5 times in hog embryo kidney cell culture. After secondary stabilization of cultured virus was achieved by means of a single passage through a baby pig, blood from this pig was used as a source of virus for adaptation to transplanted cells. Injection of this virus immunized baby hogs against subsequent infection by crude virus. (Author)

Descriptors :   (*CHOLERA, VIRUSES), SWINE, ATTENUATION, TISSUE CULTURE, IMMUNITY, TISSUE CULTURE CELLS, EMBRYOS, IMMUNITY, STABILIZATION, VACCINES, USSR

Subject Categories : Animal Husbandry and Veterinary Medicine
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE