Accession Number : AD0685805

Title :   THE CONSTANCY OF THE MOUSE UNIT IN THE BIOASSAY OF TYPE A BOTULINUM TOXIN.

Descriptive Note : Technical rept. Apr 63-Dec 67,

Corporate Author : EDGEWOOD ARSENAL MD

Personal Author(s) : Biskup,Ronald K. ; Snodgrass,Hubert L. , Jr. ; Vocci,Frank J.

Report Date : FEB 1969

Pagination or Media Count : 35

Abstract : The effects of injection volume, body weight, and environmental temperature on the toxicity of botulinum toxin Type A in mice were studied. These parameters and sex were evaluated in quantal and graded response assays. For a more sensitive method of determining toxicity, an attempt was made to utilize the hypersensitivity animal technique of Matveev in both rats and rabbits. It was concluded that the bioassay of botulinum toxin Type A in mice by both quantal and graded responses is highly reproducible. Injection volume (within limits), sex and body weight of mice are less important than environmental temperature in obtaining good reliability. Acclimation of mice for a period of 72 hours at 76 F produces the most consistent results for both types of assay. The hypersensitive animal (rabbit) technique is too erratic for assay purposes. Clinical-chemical and histological assays are insensitive in rats. (Author)

Descriptors :   (*CLOSTRIDIUM BOTULINUM, TOXINS AND ANTITOXINS), (*TOXINS AND ANTITOXINS, BIOASSAY), INJECTIONS(MEDICINE), TOXICITY, DOSAGE, SENSITIVITY, BODY WEIGHT, ALLERGIC DISEASES, RESPONSE(BIOLOGY), TEMPERATURE, ACCLIMATIZATION, RABBITS, MICE

Subject Categories : Toxicology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE