Accession Number : AD0686199

Title :   THE ROLE OF CALCIUM IN THE MITOTIC STIMULATION OF RAT THYMOCYTES BY DETERGENTS, AGMATINE AND POLY-L-LYSINE,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF RADIATION BIOLOGY

Personal Author(s) : Whitfield,J. F. ; Perris,A. D. ; Youdale,R.

Report Date : 20 MAR 1968

Pagination or Media Count : 11

Abstract : Low concentrations of the detergents cetyltrimethyl ammonium bromide, dodecyl sodium sulphate, and Triton X-100 stimulate mitotic activity in rat thymocyte populations maintained in vitro. These compounds have no mitotic effect on cells maintained in calcium-free medium. The maximum mitotic activity in the presence of a detergent such as dodecyl sodium sulphate occurs when the CaCl2 concentration is 0.04 mM while the same activity in detergent-free medium occurs only when the CaCl2 concentration is 2.5 mM. Similarly, the organic cations agmatine and poly-L-lysine also stimulated mitosis in calcium-containing medium, but not in calcium-free medium. It is concluded that all these compounds stimulate mitosis only indirectly by sensitising the mitotically competent segment of a thymocyte population to the action of calcium. (Author)

Descriptors :   (*CALCIUM, MITOSIS), (*MITOSIS, STIMULATION(PHYSIOLOGY)), CELL DIVISION, CELL STRUCTURE, CONCENTRATION(CHEMISTRY), IN VITRO ANALYSIS, CELLS(BIOLOGY), CLEANING COMPOUNDS, IONS, SENSITIVITY, CULTURE MEDIA, RATS, CANADA, PEPTIDES, GUANIDINES

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE