Accession Number : AD0686201

Title :   THE EFFECTS OF ANOXIA ON THE NUCLEAR MORPHOLOGY OF THYMUS CELLS IN NORMAL AND IRRADIATED RATS,

Corporate Author : NATIONAL RESEARCH COUNCIL OF CANADA OTTAWA (ONTARIO) DIV OF RADIATION BIOLOGY

Personal Author(s) : Whitfield,J. F. ; Perris,A. D. ; Youdale,T.

Report Date : 18 JUN 1968

Pagination or Media Count : 10

Abstract : Incubation of thymocytes in the anoxic thymus of the dead rat for periods longer than 30 min damages them. This damage causes no visible change in nuclear structure if the cells remain in the anoxic thymus, but when they are released therefrom and suspended in vitro their nuclei become structurally homogeneous ('pycnotic'). The fraction of cells which subsequently lose their nuclear structure in vitro is proportional to the concentration of inorganic phosphate in the medium. The amount of damage inflicted upon the cells during incubation in the anoxic thymus can be reduced by introducing tris(hydroxymethyl)aminomethane (Tris) buffer into the animal before its death. The establishment of primary radiation damage in thymocytes can be considerably reduced by incubation in the anoxic thymus of a Tris-treated, dead aminal during the first 2 h after irradiation. The development of radiation damage cannot be reduced if the 2-h sojourn in the anoxic thymus is delayed until 30 min after irradiation. The beneficial effect of postirradiation oxygen deprivation is at least equal to that produced by the classical 'oxygen effect'. (Author)

Descriptors :   (*CELLS(BIOLOGY), ANOXIA), IN VITRO ANALYSIS, NUCLEI(BIOLOGY), CONCENTRATION(CHEMISTRY), CULTURE MEDIA, PHOSPHATES, RADIATION EFFECTS, OXYGEN, RATS

Subject Categories : Biology
      Radiobiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE